Microaerophilic cultivation

AnaeroPack™- Anaero, AnaeroPouch™- Anaero work as oxygen absorber-CO₂ generator for Microaerophilic cultivation.

Only to open the aluminum pack and to put the sachet into the container. No need to add water. No need to use catalyst.

for Jar / AnaeroPack™- MicroAero

Use with anaerobic jars or W-Zip Standing Pouch with Petri Dish Rack.

Photo: for Jar / AnaeroPack™- MicroAero

O₂ concentration 6-12%, CO2 concentration 5-8%. Creates microaerophilic atmosphere within 1 hour!

O₂: Maintains 6-12% which is suitable for cultivation: Microaerophiles such as Campylobacter, Helicobacter will easily become extinct at 15% or higher oxygen concentration. And if less than 5%, their colony size will become smaller. Pack-MicroAero absorbs O₂ inside the container quickly and its concentration will become 6-12% within 1 hour. So microaerophiles will grow smoothly.

CO₂ : Less than 10% which may not affect pH: Carbon dioxide concentration which needs to cultivate microaerophiles is more than 5%. Higher CO₂ does not make much difference for cultivation results. On the contrary, dissolved CO₂ might lower pH of the media and have a bad influence on the susceptibility test of some antibiotics. Pack-MicroAero will prevent this by keeping CO₂ concentration about 5-8%.

Use with Pouch-Bag or W-Zip Pouch.
For 2 petri dishes. To cultivate one dish, put one empty dish to adjust the volume of the air.

AnaeroPouch™- MicroAero

Photo: AnaeroPouch™- MicroAero (for pouch)

Use with Pouch-Bag

AnaeroPouch™-MicroAero

Photo: AnaeroPouch™-MicroAero (for thin-type Jar)

for Thin-Type Jar

Campylobacter and Helicobacter prefer high humidity.
For example, put water into the jar (about 20cc) by wet cotton wool or wet filter paper. 2. Prevent surface of cultures from drying.
Pay attention not to touch sachet and the water put in jar. Rectangular jars with compartments are suitable.

	AnaeroPack™- MicroAero [for Jar]	AnaeroPouch™- MicroAero [for Pouch Bag, Thin-Type Jar]
Culture use	for Campylobacters, Helicobacters
Amount	Rectangular jar(standard) : 1 AnaeroPack™-MicroAero Rectangular jar(Large) : 1 AnaeroPack™-MicroAero(7L)	Pouch : 1 sachet [AnaeroPouch™-MicroAero for Pouch] Thin-Type Jar : 1 sachet [MicroAero for Thin-Type Jar] Use AnaeroPouch™-MicroAero for Pouch to Pouch Bag. Use AnaeroPouch™-MicroAero for Thin-Type Jar with Thin-Type Jar. These 2 sachets save not compatible.
Attention for use	These AnaeroPack™s should be used in jars, not in pouches.	Cannot be used except for Pouch or Thin-Type jar.
Attention for use	Oxygen absorption starts immediately when the aluminum sachet is opened. After opening the aluminum bag, cover up the jar quickly (within one minute for jar, within 30 seconds for pouch). Will generate CO₂ slightly less than absorbed O₂. Inner pressure of the Jars will be reduced slightly which may affect the opening of the lids.

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